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Knoepfler, Paul

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<Paul Knoepfler
Title:
Professor
Office Address: 2425 Stockton Blvd
Sacramento, CA 95817
Office Phone:
(916) 453-2289
E-Mail Address: knoepfler (at) ucdavis.edu
Education: B.A. English Literature, Reed College, Portland, OR 1989
Ph.D. Molecular Pathology, UCSD, Mark Kamps Lab 1998
Post-Doc, Bob Eisenman Lab, Fred Hutchinson CRC
Research Papers: Click Here for Research Paper Links

Research Interests:

Enhancing our understanding of the role of stem cells in tissue growth, especially that of the nervous system, and developing safe and effective regenerative medicine therapies for Shriners Patients

Current Research:

WHAT CONTROLS STEM CELL BIOLOGICAL BEHAVIOR? We are interested in answering this question about stem cells both during normal embryonic development but also during healing and regeneration. Further, we are analyzing how this control system goes awry during diseases such as developmental disorders and cancer. Our lab studies the molecular programming of embryonic and neural stem cells as well as cancer stem cells. For example, we are currently identifying the molecular mechanisms by which stem cells influence brain growth.

MYC IN STEM CELLS. We have found that a particular group of proteins called the Myc family appear to be master regulators of stem cell function. These Myc proteins are not only of critical importance for the ability of stem cells to do their normal "job" but also when the Myc genes are present at too high a concentration in stem cells they cause many human cancers. However we don't have a clear understanding of how Myc genes control the normal function of stem cells in development and regeneration nor how an excess of Myc leads to cancer. Therefore studying Myc's function in stem cells may provide critical clues as to how stem cells can be used in regenerative medicine as well as how to do our best to avoid cancer as a side effect from stem cell based regenerative medicine. Finally, these studies of Myc function should also help us to better understand human cancer in a more general sense, hopefully moving us one step closer to novel cures and prevention methods.

IPS CELLS. A final area of interest are induced pluripotent stem (iPS) cells, which represent a powerful new approach for future treatments of Shriners patients. iPS cells are a major advance in regenerative medicine, but many questions remain about these cells in terms of their properties, both positive and negative such as tumorigenicity. We are focused on addressing these and related open questions.

For more information please visit the Knoepfler lab homepage at: Knoepfler Lab Homepage
and my lab blog homepage at: Knoepfler Lab Blog Homepage


Noteworthy Publications:

TITLE
AUTHOR(s)
YEAR
DOWNLOAD
Selling Stem Cells in the USA: Assessing the Direct-to-Consumer Industry. Cell Stem Cell. 2016 Jun 29. pii: S1934-5909(16)30157-6. doi: 10.1016/j.stem.2016.06.007. [Epub ahead of print] PubMed PMID: 27374789. Turner L, Knoepfler P. 2016
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Review of Stem Cell Dialogues by Sheldon Krimsky(1). Am J Bioeth. 2016 Apr;16(4):W12-3. doi: 10.1080/15265161.2016.1145756. Knoepfler PS. 2016


When patients reach out, scientists should reach back carefully. Nat Med. 2016 Mar 3;22(3):230. doi: 10.1038/nm0316-230. Knoepfler PS. 2016
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Reviewing post-publication peer review. Trends Genet. 2015 May;31(5):221-3. doi: 10.1016/j.tig.2015.03.006. Epub 2015 Apr 4. Knoepfler P. 2015
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From bench to FDA to bedside: US regulatory trends for new stem cell therapies. Adv Drug Deliv Rev. 2015 Mar;82-83C:192-196. doi: 10.1016/j.addr.2014.12.001. Epub 2014 Dec 7. Review. Knoepfler PS. 2015
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Histone H3.3 regulates dynamic chromatin states during spermatogenesis. Development. 2014 Aug 19. pii: dev.106450. [Epub ahead of print] Yuen BT, Bush KM, Barrilleaux BL, Cotterman R, Knoepfler PS. 2014
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Miz-1 Activates Gene Expression via a Novel Consensus DNA Binding Motif.. Barrilleaux BL, Burow D, Lockwood SH, Yu A, Segal DJ, Knoepfler PS. PLoS One. 2014 Jul 1;9(7):e101151. doi: 10.1371/journal.pone.0101151. eCollection 2014. 2014
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Epigenetic mechanisms of tumorigenicity manifesting in stem cells.. Oncogene. 2014 Jun 16;0. doi: 10.1038/onc.2014.172. [Epub ahead of print] Tung PY, Knoepfler PS. 2014
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Histone H3.3 Mutations: A Variant Path to Cancer. Cancer cell 24 (5), 567-574. BTK Yuen, PS Knoepfler. 2013
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Identification of DPPA4 and DPPA2 as a Novel Family of Pluripotency‐Related Oncogenes. STEM CELLS 31 (11), 2330-2342. PY Tung, NV Varlakhanova, PS Knoepfler. 2013
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Key Action Items for the Stem Cell Field: Looking Ahead to 2014. Stem cells and development. PS Knoepfler. 2013
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Scientists: you really need to get out of the lab more. Nat Med. 2013 Sep 6;19(9):1086. doi: 10.1038/nm0913-1086. Knoepfler PS. 2013
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Chromatin immunoprecipitation assays for myc and N-myc. The Myc Gene, 117-133. Barrilleaux BL, Cotterman R, Knoepfler PS. 2013
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Automation of Library Preparation for High-resolution ChIP-seq Profiling. I Journal of Biomolecular Techniques. 2013. JBT 24 (Suppl), S45. M Henry, R Cotterman, P Knoepfler, L Comai, RW Kim, H O'Geen. 2013
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Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine zipper domain. BBRC 2013 In Press. Laskowski AI and Knoepfler PS. 2013
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Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development. Epigenetics Chromatin. 2013 Apr 9;6(1):7. Bush KM, Yuen BT, Barrilleaux BL, Riggs JW, O Geen H, Cotterman R, Knoepfler PS. 2013
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Induced pluripotency and oncogenic transformation are related processes. Stem Cells & Dev. 2013 Jan 1;22(1):37-50. Riggs JW,
Barrilleaux B,
Varlakhanova N,
Bush K,
Chan V,
and Knoepfler PS. 2013
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Call for fellowship programs in stem cell-based regenerative and cellular medicine: new stem cell training is essential for physicians. Regen Med. 2013 Mar;8(2):223-5. Knoepfler PS. 2013
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Induced pluripotent stem cells show metabolomic differences to embryonic stem cells in polyunsaturated phosphatidylcholines and primary metabolism. PLoS One 2012;7(10):e46770. *co-corresponding authors. Meissen JK , Yuen BTK, Kind T, Riggs JW, Barupala DK, Knoepfler PK*, and Fiehn O*. 2012
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Utf1: Goldilocks for ESC bivalency. Cell Stem Cell 2012; Dec 7;11(6):732-4. Laskowski AI and Knoepfler PS. 2012
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Constitutive gray hair in Mice induced by Melanocyte-specific deletion of c-Myc. 2012 Pigment Cell Melanoma Res. 2012 Mar 15. Pshenichnaya I, Schouwey K, Armaro M, Larue L, Knoepfler PS, Eisenman RN, Trumpp A, Delmas V, and Beermann F. 2012
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N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells. PLoS One. 2012;7(6):e39456. Martínez-Cerdeño V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, and Knoepfler PS. 2012
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Key anticipated regulatory issues for clinical use of human induced pluripotent stem cells. Regenerative Medicine. 2012. 2012; Sep;7(5):713-20. Knoepfler PS. 2012
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N-myc controls proliferation, morphogenesis and patterning of the inner ear. J. NeuroSci. 2011 31(19):7178-89. Dominguez-Frutos E., Lopez Hernandez I., Vendrell V., Gallozzi M., Gutsche K., Sharpe J., Knoepfler PS, Eisenman RN, Trumpp A., and Schimmang T. 2011
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Overexpression of oncogenes in human cells using ecotropic lentivirus for enhanced biosafety. J Vis Exp. 2011 Jul 24;(53). Barrilleaux B. and Knoepfler PS. 2011

A yearlong experiment in academic stem cell blogging. 2011. Nature. In Press. Knoepfler PS. A yearlong experiment in academic stem cell blogging. 2011. Nature. In Press. 2011
In Press
Inducing iPS cells to escape the dish. 2011. Cell Stem Cell. In Press. Barrilleaux B. and Knoepfler PS. 2011
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Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells. Epigenetics & Chromatin. 2011 Nov 4;4(1):20. Varlakhanova NV, Cotterman RF, Bradnam K, Korf I, and Knoepfler PS. 2011
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My Year As A Stem Cell Blogger. Nature. 475(7357):425 Knoepfler PS. 2011
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IPRM
2425 Stockton Blvd.
Sacramento, CA 95817
Tele: (916) 453-2227
Fax: (916) 453-2288
webmaster (at) iprmd.org